Ngokufana nabacwangcisi basezidolophini bacwangcisa ngononophelo ukuhamba kwezithuthi kumbindi wedolophu, iiseli zilawula ngobuchule intshukumo yeemolekyuli ukunqumla imida yazo yenyukliya. Isebenza njengabagcini-masango be-microscopic, ii-nuclear pore complexes (NPCs) ezifakwe kwinwebu yenyukliya zigcina ulawulo oluchanekileyo kolu rhwebo lwemolekyuli. Umsebenzi owothusayo ovela eTexas A&M Health utyhila ukukhetha okuphucukileyo kwale nkqubo, okunokuthi kubonelele ngemibono emitsha malunga nokuphazamiseka kwe-neurodeergenerative kunye nophuhliso lomhlaza.
Ukulandela umkhondo weeNdlela zeMolekyuli
Iqela lophando likaGqr. Siegfried Musser kwiKholeji yezoNyango yaseTexas i-A&M yeKholeji yezoNyango iye yaphayona uphando kuhambo olukhawulezayo, olungangqubaniyo lweemolekyuli ngomqobo we-nucleus ye-double-membrane barrier. Upapasho lwabo olubalulekileyo lweNdalo lunika iinkcukacha ngeziphumo eziguqukileyo ezenziwe zaba nokwenzeka yitekhnoloji ye-MINFLUX-indlela yokucinga ephucukileyo ekwaziyo ukubamba iintshukumo zemolekyuli ze-3D ezenzeka kwi-milliseconds kwizikali ezimalunga ne-100,000 ngokuphindwe kancinci kunobubanzi beenwele zomntu. Ngokuchaseneyo neengqikelelo zangaphambili malunga neendlela ezahluliweyo, uphando lwabo lubonisa ukuba iinkqubo zenyukliya zokungenisa nokuthumela ngaphandle zabelana ngeendlela ezithungelanayo ngaphakathi kwesakhiwo se-NPC.
UkuFumana okumangalisayo umngeni kwiiModeli esele zikho
Uqwalaselo lweqela luveze iipatheni zendlela ezingalindelekanga: iimolekyuli zihamba macala omabini kwiitshaneli ezicutheneyo, zijikeleza enye kwenye kunokulandela iindlela ezizinikezeleyo. Okuphawulekayo kukuba, la maqhekeza agxininise kufuphi neendonga zesiteshi, eshiya indawo ephakathi engenamntu, ngelixa inkqubela yabo iyancipha ngokukhawuleza - malunga namaxesha e-1,000 acothayo kunokunyakaza okungathintekiyo - ngenxa yenethiwekhi yeprotheyini ephazamisayo eyenza indawo ene-syrupy.
U-Musser uyichaza le nto "njengeyona meko ingumceli mngeni yendlela onokuthi uyicinge - ukuhamba ngeendlela ezimbini kwiipaseji ezimxinwa." Uyavuma, “Izinto esizifumeneyo zibonisa indibaniselwano ebingalindelekanga, ebonisa ukuntsonkotha okukhulu kuneengcamango zethu zantlandlolo.”
Ubuchule Naphezu Kwemiqobo
Okubangela umdla kukuba, iinkqubo zezothutho ze-NPC zibonisa ukusebenza kakuhle okumangalisayo nangona le miqobo. UMusser uqikelela ukuba, "Ubuninzi bendalo be-NPC bunokuthintela ukusebenza ngokugqithisileyo, ngokufanelekileyo ukunciphisa ukuphazamiseka kokhuphiswano kunye nemingcipheko yokuvala." Olu phawu loyilo lwendalo lubonakala luthintela ukuvalwa kwegridi yemolekyuli, Apha'inguqulelo ebhalwe ngokutsha ene-syntax eyahluka-hlukeneyo, ulwakhiwo, kunye nokwaphulwa kwemihlathi ngelixa kugcinwe intsingiselo yoqobo:
I-Molecular Traffic ithatha indlela: ii-NPC zityhila iindlela ezifihlakeleyo
Endaweni yokuba uhambe ngqo kwi-NPC'I-axis esembindini, iimolekyuli zibonakala zihamba ngendlela enye yezibhozo ezikhethekileyo zothutho, nganye ivaleleke kwisakhiwo esithetha-esifana ecaleni kwepore.'s umsesane wangaphandle. Olu lungelelwaniso lwesithuba lucebisa indlela yoyilo esisiseko enceda ukulawula ukuhamba kwemolekyuli.
U-Musser uyacacisa,“Ngelixa igwele imingxunya yenyukliya zaziwa ukuba ziqulathe a'iplagi esembindini,'eyona ndlela ibunjwe ngayo ihlala iyimfihlelo. Kwiiseli zomntu, olu phawu hasn't ibonwe, kodwa ukwahlulahlula okusebenzayo kuyavakala-kunye ne pore's iziko linokusebenza njengendlela ephambili yokuthumela ngaphandle ye-mRNA.”
Uqhagamshelwano lweSifo kunye neMingeni yoNyango
Ukungasebenzi kwi-NPC-isango elibalulekileyo leselula-ubotshelelwe kwiziphazamiso ezinzima zemithambo-luvo, kuquka i-ALS (Lou Gehrig's isifo), i-Alzheimer's, kunye Huntington's isifo. Ukongeza, ukunyuka komsebenzi wokurhweba nge-NPC kunxulunyaniswa nokuqhubekeka komhlaza. Nangona ukujolisa kwimimandla ethile yepore kunokunceda ngokwethiyori ukuvalwa kokuvalwa okanye ukucotha uthutho olugqithisileyo, uMusser ulumkisa ngelithi ukuphazamisana nomsebenzi we-NPC kunomngcipheko, ngenxa yendima yako esisiseko ekusindeni kweeseli.
“Kufuneka sahlule phakathi kweziphene ezinxulumene nezothutho kunye nemiba ebotshelelwe kwi-NPC'indibano okanye ukuchithwa,”uyaphawula.“Ngelixa uninzi lwezonxibelelwano lwezifo lunokwenzeka luwela kudidi lokugqibela, ngaphandle zikhona-njengotshintsho lwemfuza lwe-c9orf72 kwi-ALS, edala imidibaniso ethintela i pore ngokwasemzimbeni.”
Izalathiso zexesha elizayo: Imephu yeNdlela zeMpahla kunye neMifanekiso yeSeli ephilayo
Musser kunye nomdibanisi uGqr Abhishek Sau, osuka eTexas A&M's Joint Microscopy Lab, isicwangciso ukuphanda ukuba iintlobo impahla ezahlukeneyo-ezifana ne-ribosomal subunits kunye ne-mRNA-landela iindlela ezizodwa okanye ukudibana kwiindlela ekwabelwana ngazo. Umsebenzi wabo oqhubekayo kunye namaqabane aseJamani (i-EMBL kunye ne-Abberior Instruments) inokuphinda ilungelelanise i-MINFLUX yomfanekiso wexesha lokwenyani kwiiseli eziphilayo, inika iimbono ezingazange zibonwe ngaphambili zothutho lwenyukliya.
Ixhaswa yinkxaso-mali ye-NIH, olu phononongo luhlaziya ukuqonda kwethu ukuhanjiswa kweselula, kubonisa indlela ii-NPCs ezigcina ngayo ucwangco kwimetropolis ephithizelayo ye-nucleus.
Ixesha lokuposa: Mar-25-2025
